Launch from Cape Canaveral (KSC); landing on Cape Canaveral (KSC).

It was the 9th and final MIR-Docking mission. Following a two day solo flight the Discovery docked with the MIR space station on 04.06.1998. Common flight with the 25th MIR resident crew (04. - 08.06.1998). Supplies and equipment were brought to MIR and several experiments were brought from the MIR back to Earth. Andrew Thomas (final U.S. astronaut on MIR) returned to Earth with the Discovery. The astronauts and cosmonauts performed an experiment to find the leak in the module Spektr, but this failed.

Several secondary payloads: The Space Acceleration Measurement System (SAMS) and the tissue engineering co-culture (COCULT) investigations, as well as two crystal growth experiments. A prototype of the Alpha Magnetic Spectrometer (AMS) was carried. It was designed to look for dark and missing matter in the universe.