Launch from Cape Canaveral (KSC); landing on Cape Canaveral (KSC). Fourth Space Station Assembly Mission ISS-03-2A.2B. Space Station assembly flight ISS-2A.2b utilized the SPACEHAB Double Module and the Integrated Cargo Carrier (ICC) to bring supplies to the station.

Following a two day solo flight the Atlantis docked with the ISS on 10.09.2000.

EVA by Lu and Malenchenko on 11.09.2000 (6h 15m) to complete final connections (electrical-, data- and television cables) between Zvezda module and component Zarya. A magnometer was boomed to a port of Zvezda module. The magnetometer serves as a three-dimensional compass designed to minimize Zvezda propellant usage by relaying information to the module's computers regarding its orientation relative to the Earth.

On flight day four the crew entered the International Space Station through Pressurized Mating Adapter-2 (PMA-2) to begin the transfer operations of more than three tons of hardware and supplies. Transfer of supplies and maintenance tasks continued well into the fifth day, while orbiter consumables remained above the required levels allowing managers to extend the mission one additional day. Finally they placed two Russian Orlan space suits near the air lock.