Launch from Cape Canaveral (KSC); night landing on Cape Canaveral (KSC). The launch was delayed three times due to high winds. Third Space Station Assembly mission ISS-03-2A.2A.

Following a two day solo flight the Atlantis docked with the ISS on 20.05.2000. The primary mission objectives for STS-101 was to deliver supplies to the International Space Station, with the supplies hauled up using a Spacehab double module and Spacehab Integrated Cargo Carrier pallet, perform a spacewalk and then reboost the station from 230 to 250 statute miles (370 to 402 km).

Voss and Williams performed an EVA on 21.05.2000 (6h 44m). They installed the final parts of the Russian built crane, replaced a faulty antenna and installed some handrails and a camera cable.

The crew had to do several repairing work (replacemt of batteries) in the ISS.

This mission was almost similar to the Columbia disaster. A damaged tile seam caused a breach which allowed superheated gas to enter the left wing during reentry. The gas did not penetrate deeply and the damage was repaired before the next flight. If it had penetrated deeply the Shuttle could have been destroyed during reentry.