Launch from Cape Canaveral (KSC); landing on Cape Canaveral (KSC).

Mission "US Microgravity Payload-3" carried several experiment in different sientific fields. The USMP-3 payload consisted of four major experiments mounted on two Mission Peculiar Experiment Support Structures (MPESS) and three Shuttle Mid-deck experiments. The experiments were: Advanced Automated Directional Solidification Furnace (AADSF), Material pour l'Etude des Phenomenes Interessant la Solidification sur Terre et en Orbite (MEPHISTO), Space Acceleration Measurement System (SAMS), Orbital Acceleration Research Experiment (OARE), Critical Fluid Light Scattering Experiment (ZENO) and Isothermal Dendritic Growth Experiment (IDGE). But the primary objective of STS-75 was to carry the Tethered Satellite System Reflight (TSS-1R) into orbit and to deploy it spaceward on a conducting tether. The deploying of the Italian Tethered Satellite System-1 failed, because the tether has broken after a distance of 19 km. The satellite was lost.

The mission was extended one day due of bad weather at Cape Canaveral (KSC).