Launch from Cape Canaveral (KSC); landing at Cape Canaveral (KSC); ISS-20A Tranquility (formerly: NODE 3), Cupola.

The first launch attempt was scheduled for February 07, 2010, but was scrubbed due to clouds over the Kennedy Space Center.

Space Shuttle Endeavour's primary payloads were the Tranquility module and the Cupola, a robotic control station with six windows around its sides and another in the center, providing a 360-degree view around the station.

Following a two day solo flight Endeavour docked to the ISS on February 10, 2010. Joint operations with the ISS expedition 22 were performed.

During the first part of the crew's workday, they performed a series of burns to catch up and dock with the International Space Station (ISS). Once the shuttle was 600 feet (180 m) below the ISS, commander George Zamka began what is known as the Rendezvous Pitch Maneuver (RPM). During the maneuver, ISS commander Jeffrey Williams and flight engineer Oleg Kotov took photos of the shuttle's thermal protection system (TPS).

The first EVA by Robert Behnken and Nicholas Patrick occured on February 12, 2010 (6h 32m) to remove a cover that has been in place to protect a port on the Unity node, the location where Tranquility was attached robotically halfway through the spacewalk. Next, the spacewalkers removed and stored a spare parts platform from the Special Purpose Dexterous Manipulator, or DEXTRE, a two-armed space station robot capable of handling delicate assembly tasks currently performed by spacewalkers. Finally, they installed four electronics systems cables between Unity and Tranquility to bring the new node to life.

From inside the station, Terry Virts and Kathryn Hire used the station's robotic arm to remove Tranquility from the shuttle's payload bay, then carefully maneuver then installed the node onto the port side of the Unity node. Tranquility was built for NASA by Thales Alenia Space in Turin, Italy, under contract to the European Space Agency.

The second EVA was again performed by Robert Behnken and Nicholas Patrick on February 14, 2010 (5h 54m) to install ammonia plumbing connectors between Unity and Tranquility and covering them with thermal insulation. When turned on, the ammonia will provide cooling to Tranquility. Then they prepared a port on the Earth facing side of Tranquility for the flight day 8 relocation and attachment of the Cupola.

The Cupola was detached from the front port of Tranquility on flight day 8 and relocated to Tranquility nadir. The Cupola is 4.9 feet in length, 9.7 feet in diameter and has a weight of about 4,145 pounds in orbit. The Cupola includes window shutters that provide orbital debris protection when closed.

On February 16, 2010 Nicholas Patrick and Robert Behnken, together with expedition 22 crew members Jeffrey Williams and Soichi Noguchi, maneuvered the Pressurized Mating Adapter 3 from its location on the Harmony module to the open port on the end of Tranquility.

The third and final EVA by Robert Behnken and Nicholas Patrick occured on February 17, 2010 (5h 48m) to turn on the ammonia cooling lines between Unity and Tranquility, installing heater and data cables on Tranquility, removing insulation and launching locks from the newly-installed Cupola, and installed handrails on the outside of Tranquility.