Launch from Cape Canaveral (KSC); landing on Edwards AFB; ISS LF1/MPLM 2-03. It was the first shuttle launch since 907 days.

STS-114 marked the return to flight of the Space Shuttle after the Columbia disaster and was the second Shuttle flight with a female commander (Eileen Collins).

Docking with ISS on 28.07.2005 and common work with the ISS expedition 11. The STS-114 mission delivered supplies to the International Space Station. However, the major focus of the mission was testing and evaluating new Space Shuttle flight safety techniques, which included new inspection and repair techniques. The crewmembers used the new Orbiter Boom Sensor System (OBSS) - a set of instruments on a 50-foot (15 m) extension attached to the Canadarm.

First EVA by Robinson and Noguchi on 30.07.2005 (6h 50m) for testing thermal protection system repair techniques and installation of the External Stowage Platform-2 Attachment Device (ESPAD) on the space station.

On July 30, 2005, NASA announced that STS-114 would be extended for one day.

Second EVA by Robinson and Noguchi on 01.08.2005 (7h 14m) to replace a failed International Space Station attitude control gyroscope.

Third and final EVA by Robinson and Noguchi on 03.08.2005 (6h 01m) to attach a massive external tool box to the space station's Quest airlock module, mounting an experiment package on the top of the station's solar array truss, and remove two "gap fillers" sticking up between heat shield tiles on Discovery's belly.

The mission was extended for another day due of bad weather in Florida and was diverted to the Edwards AFB.