Launch from Cape Canaveral (KSC); HST-SM-04; landing on the Edwards AFB.

Capture of the Hubble Space Telescope and first EVA by Grunsfeld and Feustel on 14.05.2009 (7h 20m) to replace a wide field camera. They also swapped out three batteries and installed a mechanism for a spacecraft to capture for de-orbit at the end of the telescope's life.

Second EVA by Massimino and Good on 15.05.2009 (7h 56m) to change out three boxes, each containing two of the telescope's six gyroscopes, and three batteries.

Third EVA by Grunsfeld and Feustel on 16.05.2009 (6h 36m) to install the Cosmic Origins Spectrograph, a device that will perform spectroscopy, the science of breaking up light into its individual components. The astronauts also conducted repairs to the Advanced Camera for Surveys, which has experienced power failures, causing two of the telescope's three observing channels to stop operating.

Fourth EVA by Massimino and Good on 17.05.2009 (8h 02m) to repaire and upgrade the Space Telescope Imaging Spectrograph, which stopped working in 2004 due to power failures, and install a stainless steel blanket on Hubble's exterior. The blankets provide additional thermal protection for some equipment bays, replacing the existing multi-layer insulation that has degraded over time.

Fifth and final EVA by Grunsfeld and Feustel on 18.05.2009 (7h 02m) to replace a sensor needed for pointing the telescope and install another blanket on Hubble's exterior.

The landing was delayed two days due of bad weather in Florida.