Launch from Cape Canaveral (KSC); landing on Edwards AFB. It was the first night launch and first night landing of a Space Shuttle.

The primary payload was INSAT-1B, an Indian communications and weather observation satellite, which was released by the orbiter and boosted into a geostationary orbit. The secondary payload, replacing a delayed NASA communications satellite, was a four-metric-ton dummy payload, intended to test the use of the shuttle's "Canadarm" remote manipulator system. Scientific experiments carried on board Challenger included the environmental testing of new hardware and materials designed for future spacecraft, the study of biological materials in electric fields under microgravity, and research into space adaptation syndrome (also known as "space sickness"). The flight furthermore served as shakedown testing for the previously launched TDRS-1 satellite, which would be required to support the subsequent STS-9 mission.

While on orbit, Challenger made a number of altitude and attitude adjustments, in order to test the behavior of a Shuttle orbiter and to perform some experiments in different thermal conditions. By exposing or shading areas from the sun in an unusual way, it was possible to induce particularly warm or cold conditions and observe any resulting problems