Launch from Cape Canaveral (KSC); landing on Cape Canaveral (KSC). Mission ISS-10-7A Joint Airlock. Primary objectives were to install the Quest Joint Airlock. The Joint Airlock is 20 ft (6.1 m) long, 13 ft (4.0 m) in diameter and weighs 6.5 short tons (5.9 metric tons). The Joint Airlock is a pressurized flight element consisting of two cylindrical chambers attached end-to-end by a connecting bulkhead and hatch. It should give the ISS resident crews the possibility to perform EVAs without using a Space Shuttle airlock.

Following a two day solo flight the Atlantis docked with the ISS on July 14, 2001 and common work with the ISS expedition 2 was done.

The first EVA was performed by Michael Gernhardt and James Reilly on July 14, 2001 (5h 59m) to install Joint Airlock Module ("Quest") and attaching two high pressure oxygen tanks.

The second EVA was conducted by Michael Gernhardt and James Reilly on July 18, 2001 (6h 29m) to mount one oxygen and one nitrogen tank.

The third an final EVA (using first time the new airlock Quest) was again performed by Michael Gernhardt and James Reilly on July 20, 2001 (4h 02m) to attach the final nitrogen tank.