They installed a power extension cable between the Unity and Tranquility nodes to provide a contingency power source should it be required. The spacewalkers moved the failed ammonia pump module that was replaced in August 2010 from an attachment bracket to a stowage platform adjacent to the Quest airlock. Drew and Bowen installed a piece of hardware that will go under a camera on the truss. The hardware will tilt the camera to provide clearance for a spare part to be installed on a future mission. They next replaced a guide for the rail cart system used for moving cargo along the truss. The guides were removed when the astronauts were performing work on the station's starboard Solar Alpha Rotary Joint, which rotates the solar arrays to track the sun.

The astronauts removed thermal insulation from a platform, while Bowen swaped out an attachment bracket on the Columbus module. Bowen then installed a camera assembly on the Dextre robot then removed insulation from Dextre's electronics platform. Drew installed a light on a cargo cart and repair some dislodged thermal insulation from a valve on the truss then remove other insulation from Tranquility. The final task was be to "fill" a special bottle with space for a Japanese education payload. The bottle will be part of a museum exhibit for public viewing.